1. Classify Microbes ?
  2. Write an account on staining techniques ?
  3. Write briefly about differential staining techniques ?
  4. What are the isolation techniques used for micro organisms ? Explain in detail ?


  1. On the basis of shape bacteria are classified how many major types?

A. Four types

B. Five types

C. Six types

D. Eight types

2. A common characteristic of archaebacteria……?

A. Structure of cell membrane

B. Structure of cell wall

C. Has fatty acid synthetase

D. Structure of flagellin protein

3. Staining material of gram positive bacterium is …….?

A. Fast green

B. Haematoxylon

C. Crystal violet

D. Safranin

4. Gram staining is an example for…….?

A. Simple staining

B. Differential staining

C. Negative staining

D. None of these  

5. If only one stain is used for staining a specimen…;

A. Simple staining

B. Negative staining

C. Differential staining

D. None of these  

6. Separation of a single colony is…..;

A. Pure-culturing

B. Isolation

C. Separation

D. Both a and b     

7. Which of the staining technique helps in demonstrating spore structure in bacteria as well as free spores?

A. Acid-fast stain

B. Endospore stain

C. Capsule stain

D. Flagella stain

8. In Gram-staining, iodine is used as a ______________?

A. Fixative

B. Mordant

C. Solublizer

D. Stain

9. The term prokaryotes refers to which of the following?

A. Very small organisms

B. Unicellular organisms that have no nucleus

C. Multicellular organisms

D. Cells that resemble animal cells more than plant cells

10. Which of the following dye is commonly used for the Negative staining technique?

A. Crystal violet

B. India ink

C. Methylene blue

D. Iodine

11. Which of the following method can be used to determine the number of bacteria quantitatively?

A. Streak-plate

B. Spread-plate

C. Pour plate

D. Pour-plate and spread plate

12. Loop wire is used in which of the following techniques?

A. Pour-plate

B. Streak-plate

C. Spread-plate

D. Roll-tube technique

Answers :

  1. A. Four
  2. C. Has fatty acid synthetase
  3. C. Crystal violet
  4. B. Differential staining
  5. A. Simple staining
  6. B. Isolation
  7. B. Endospore stain
  8. B. Mordant
  9. B. Unicellular organisms that have no nucleus
  10. B. Indian Ink
  11. D. Pour-plate and spread plate
  12. B. Streak-plate

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